4-arm 10 kda peg thiol  (Creative PEGWorks)

Journal: bioRxiv

Article Title: Designing viscoelastic mucin-based hydrogels

doi: 10.1101/656801

Figure Lengend Snippet: (A-D) Multiple particle tracking of 100-nm PEG-NP in mucin solutions with varying cross-linking polymers. Each panel represents the ensemble average mean squared displacement as function of time scale τ (MSD; ⟨Δ r ⟩) of 100 nm PEG-NP at hour 0 (triangles) and hour 9 (circles) in 2% w/v of PGM and 2% w/v of ( A ) PEG-1SH, ( B ) PEG-2SH, ( C ) PEG-4SH, and ( D ) dextran-SH. Reference lines with slope n =0.5 are included in each panel.

Article Snippet: Crosslinking reagents tested in this work include monofunctional PEG-thiol (PEG-1SH; 10 kDa), bifunctional PEG-thiol (PEG-2SH; 10 kDa), 4-arm PEG-thiol (PEG-4SH; 10 kDa), each purchased from Laysan Bio Inc. and a thiol-functionalized dextran (dextran-SH; 10 kDa) purchased from Nanocs.

Techniques:

Journal: bioRxiv

Article Title: Designing viscoelastic mucin-based hydrogels

doi: 10.1101/656801

Figure Lengend Snippet: ( A ) MSD (⟨Δ r ⟩) as a function of time scale (τ) of 100 nm PEG-NP in 2% w/v PGM and 2% w/v PEG-SH-4 solution 0 hours (downward triangles) and 9 hours (circles) after pre-treatment with iodoacetamide (IAM). ( B ) MSD as a function of τ of 100 nm PEG-NP in 2% w/v PGM solution after 9 hours (PGM; upward triangles), in 2% PGM/PEG-4SH hydrogel after 9 hours (PGM Gel; circles), and in 2% PGM/PEG-4SH hydrogel after a 30-minute treatment with N -acetyl cysteine (PGM Gel + NAC; cross).

Article Snippet: Crosslinking reagents tested in this work include monofunctional PEG-thiol (PEG-1SH; 10 kDa), bifunctional PEG-thiol (PEG-2SH; 10 kDa), 4-arm PEG-thiol (PEG-4SH; 10 kDa), each purchased from Laysan Bio Inc. and a thiol-functionalized dextran (dextran-SH; 10 kDa) purchased from Nanocs.

Techniques:

Journal: bioRxiv

Article Title: Designing viscoelastic mucin-based hydrogels

doi: 10.1101/656801

Figure Lengend Snippet: Elastic modulus G’ (circles) and viscous modulus, G” (squares) of PGM-based (A , B) and BSM-based (C , D) mucin hydrogels after a 24-hr gelation time. Data represents average of 3 independently prepared hydrogels. ( A ) Strain (γ) sweep at frequency (ω) of 1 rad/s and ( B ) frequency sweep at γ=1% for 2% PGM and 2% PEG-4SH hydrogel. At ω = 1 rad/s, we measured G ’ ∼ 244 Pa and tan δ ∼ 8.1. ( C ) Strain (γ) sweep at frequency (ω) of 1 rad/s and ( D ) Frequency sweep at γ=1% for 2% BSM and 2% PEG-4SH hydrogel. At ω = 1 rad/s, we measured G ’ ∼ 270 Pa and tan δ ∼ 1.4.

Article Snippet: Crosslinking reagents tested in this work include monofunctional PEG-thiol (PEG-1SH; 10 kDa), bifunctional PEG-thiol (PEG-2SH; 10 kDa), 4-arm PEG-thiol (PEG-4SH; 10 kDa), each purchased from Laysan Bio Inc. and a thiol-functionalized dextran (dextran-SH; 10 kDa) purchased from Nanocs.

Techniques:

Journal: bioRxiv

Article Title: Designing viscoelastic mucin-based hydrogels

doi: 10.1101/656801

Figure Lengend Snippet: (A-F) Microrheology of a 2% w/v PGM and 2% PEG-4SH gel measured using PEG-NP probes with diameters of 100 and 500 nm. Symbols in each panel are median values and bars indicate the interquartile range. ( A , B ) The MSD at τ = 1 s (⟨Δ r (τ=1 s)⟩) for (A) 100 and (B) 500 nm PEG-NP during a 9-hour gelation period. ( C , D ) Elastic to viscous moduli ratio (G’/G”) at ω = 1 rad/sec calculated from measured MSD of (C) 100 nm and (D) 500 nm PEG-NP.( E , F ) Estimated pore size based on analysis of MSD at ; black circles) and G’ at ω = 1 rad/s (ξ≈( k B T /G’) / ; red squares) for (E) 100 nm and (F) 500 nm PEG-NP probes.

Article Snippet: Crosslinking reagents tested in this work include monofunctional PEG-thiol (PEG-1SH; 10 kDa), bifunctional PEG-thiol (PEG-2SH; 10 kDa), 4-arm PEG-thiol (PEG-4SH; 10 kDa), each purchased from Laysan Bio Inc. and a thiol-functionalized dextran (dextran-SH; 10 kDa) purchased from Nanocs.

Techniques:

Journal: bioRxiv

Article Title: Designing viscoelastic mucin-based hydrogels

doi: 10.1101/656801

Figure Lengend Snippet: (A,B) Kinetics of PGM/PEG-4SH gel formation as analyzed by microrheology using 100 nm PEG-NP probes. The gelation point is measured as α=log 10 [MSD]/log 10 [τ] ≤ 0.5. Each panel displays the mean and standard error of measured α. (A) α as a function of gelation time in hydrogels with a constant PEG-4SH concentration of 2% w/v and varying PGM concentrations of 1% w/v (black), 2% w/v (red), 3% w/v (green), 4% w/v (blue), and 5% w/v (brown). (B) α as a function of gelation time in hydrogels with a constant PGM concentration of 2% w/v and varying PEG-4SH concentration of 1% w/v (black), 2% w/v (red), 3% w/v (green), 4% w/v (blue), and 5% w/v (brown).

Article Snippet: Crosslinking reagents tested in this work include monofunctional PEG-thiol (PEG-1SH; 10 kDa), bifunctional PEG-thiol (PEG-2SH; 10 kDa), 4-arm PEG-thiol (PEG-4SH; 10 kDa), each purchased from Laysan Bio Inc. and a thiol-functionalized dextran (dextran-SH; 10 kDa) purchased from Nanocs.

Techniques: Concentration Assay